Genview Products
M-MLV Reverse transcriptase
Description:
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV) uses Single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA strand. This enzyme is isolated from E coli expressing a portion of the pol gene of M-MLV on a plasmid.
Components:
M-MLV
5 × RT Buffer
Unit Definition:
Unit activity is calculated assuming a specific enzyme activity of 350,000 units per mg protein. Protein is determined by a modification of the Lowry method, using BSA as a standard.
One unit of M-MLV incorporates 1 nmol dTTP into acid-precipitable material in 10 minutes at 37
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